MAP1LC3B 蛋白, Human

MAP1LC3B is a key ubiquitin-like modifier essential for the formation of autophagosome vacuoles, which maintains cellular homeostasis. In mitophagy, it regulates mitochondrial number, suppresses reactive oxygen species and ensures energy efficiency. MAP1LC3B Protein, Human is the recombinant human-derived MAP1LC3B protein, expressed by E. coli , with tag free. The total length of MAP1LC3B Protein, Human is 125 a.a., with molecular weight of ~16.15 kDa.

MAP1LC3B 是一种泛素样修饰剂，在自噬体液泡形成中发挥着关键作用，有助于细胞稳态。它参与线粒体自噬，调节线粒体的数量和质量，防止过量的活性氧产生并确保能量效率。在细胞应激期间，它与 C-18 神经酰胺结合，将自噬溶酶体锚定在线粒体外膜上，促进受损线粒体的消除。与参与吞噬细胞膜伸长的 LC3 不同，MAP1LC3B 所属的 GABARAP/GATE-16 亚家族对于后期自噬体成熟至关重要。MAP1LC3B 通过与 TEX264 相互作用进一步促进初级纤毛发生并协调内质网子结构域重塑为自噬体。为了应对营养应激，它会招募辅助因子 JMY，增强肌动蛋白成核和自噬体生物发生。这种多功能蛋白质与多种伙伴相互作用，包括用于包涵体降解的 SQSTM1、ATG13、MAPK15、BNIP3、KEAP1、PCM1 和 TBC1D5，突出显示其参与多种细胞过程和信号通路。

Human

E. coli

Tag Free

Q9GZQ8 (M1-V125)

81631  [NCBI]

MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV

Approximately 16.15 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH 8.0 .

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.